製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号Q15078
    • 生物種Human
    • 分子量60 kDa including tags
    • 領域1 to 307

特性

Our Abpromise guarantee covers the use of ab119724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    SDS-PAGE

  • 精製度> 70 % SDS-PAGE.
    The purity was determined to be 70% by densitometry
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 25% Glycerol, 0.29% Sodium chloride, 0.79% Tris HCl, 0.003% EDTA, 0.003% DTT, 0.31% Glutathione, 0.002% PMSF

関連情報

  • 別名
    • CD5R1_HUMAN
    • CDK5 activator 1
    • CDK5P35
    • CDK5R
    • CDK5R1
    • Cyclin dependent kinase 5 regulatory subunit 1
    • Cyclin-dependent kinase 5 activator 1
    • Cyclin-dependent kinase 5 regulatory subunit 1
    • NCK 5A
    • Neuronal CDK5 activator
    • p23
    • p25
    • p35
    • Regulatory partner for CDK5 kinase
    • Tau protein kinase II 23 kDa subunit
    • TPKII regulatory subunit
    see all
  • 機能p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
  • 組織特異性Brain and neuron specific.
  • 配列類似性Belongs to the cyclin-dependent kinase 5 activator family.
  • 翻訳後修飾The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
  • 細胞内局在Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt

Recombinant Human p35 protein 画像

  • SDS Page analysis of ab119724.

Recombinant Human p35 protein (ab119724) 使用論文

ab119724 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119724.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"