Recombinant Human p27 KIP 1 protein (ab56279)

関連情報

• 別名
  • AA408329
  • AI843786
  • Cdki1b

  • CDKN 1B
  • CDKN 4
  • CDKN1B
  • CDKN4
  • CDN1B_HUMAN
  • Cyclin Dependent Kinase Inhibitor 1B
  • Cyclin dependent kinase inhibitor p27
  • Cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • Cyclin-dependent kinase inhibitor p27
  • Cyclin-dependent kinase inhibitor p27 Kip1
  • KIP 1
  • KIP1
  • MEN1B
  • MEN4
  • OTTHUMP00000195098
  • OTTHUMP00000195099
  • p27
  • p27 Kip1
  • P27-like cyclin-dependent kinase inhibitor
  • p27Kip1

  see all
• 機能Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
• 組織特異性Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
• 関連疾患Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.
• 配列類似性Belongs to the CDI family.
• ドメインA peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity.
• 翻訳後修飾Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
  Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
  Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation.
• 細胞内局在Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6 and this leads to lysosomal degradation.

• Target information above from: UniProt accession P46527 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt