Recombinant Human Osteopontin protein (ab87460)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N

特性

Our Abpromise guarantee covers the use of ab87460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度> 90 % SDS-PAGE.
    Purified using conventional chromatography.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM EDTA, 1mM DTT, pH 7.5

関連情報

  • 別名
    • BNSP
    • Bone sialoprotein 1
    • BSP I
    • BSPI
    • Early T lymphocyte activation 1
    • ETA 1
    • ETA1
    • MGC110940
    • Nephropontin
    • OPN
    • Osteopontin
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
    • OSTP_HUMAN
    • PSEC0156
    • Secreted phosphoprotein 1
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
    • SPP 1
    • SPP-1
    • SPP1
    • SPP1/CALPHA1 fusion
    • Urinary stone protein
    • Uropontin
    see all
  • 機能Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • 組織特異性Bone. Found in plasma.
  • 配列類似性Belongs to the osteopontin family.
  • 翻訳後修飾Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在Secreted.
  • Information by UniProt

Recombinant Human Osteopontin protein 画像

  • ab87460 on 15% SDS-PAGE (3µg)
    Note: molecular weight on SDS-PAGE will be shifted up.

Recombinant Human Osteopontin protein (ab87460) 使用論文

ab87460 has not yet been referenced specifically in any publications.

Product Wall


The lab let me know the following:

The band appears higher on SDS PAGE, but we haven’t verified the specific cause. As you know in SDS-PAGE the migration is also affected by other factors than size, so the actual band size observed ...

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We carry three osteopontin proteins in the catalogue.

Recombinant full length Human Osteopontin (amino acid 17-314) expressed in 293 cells, 60-65 kDa (SwissProt P10451), ab81549.

http://www.abcam.com/Osteopontin-protein-Active-ab815...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"