Recombinant Human Oct4 protein (ab95845)

製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      23 kDa
    • 領域
      1 to 212

関連製品

特性

Our Abpromise guarantee covers the use of ab95845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    Dot blot

    SDS-PAGE

    Indirect ELISA

  • 精製度
    > 90 % SDS-PAGE.
    ab95845 was refolded to soluble form.
  • 製品の状態
    Liquid
  • 備考

    This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 5% Glycerol, PBS (1.5% Monobasic dihydrogen potassium phosphate, 8.1mM Dibasic monohydrogen sodium phosphate, 500mM Sodium chloride, 2.7mM Potassium chloride, pH 7.4)

関連情報

  • 別名
    • Octamer binding transcription factor 4
    • MGC22487
    • Oct 3
    • Oct 4
    • Oct-3
    • Oct-4
    • OCT3
    • Oct4
    • Octamer binding protein 3
    • Octamer binding protein 4
    • Octamer binding transcription factor 3
    • Octamer-binding protein 3
    • Octamer-binding protein 4
    • Octamer-binding transcription factor 3
    • OTF 3
    • OTF 4
    • OTF-3
    • OTF3
    • OTF4
    • PO5F1_HUMAN
    • POU class 5 homeobox 1
    • POU domain class 5 transcription factor 1
    • POU domain transcription factor OCT4
    • POU domain, class 5, transcription factor 1
    • POU-type homeodomain-containing DNA-binding protein
    • POU5F1
    see all
  • 機能
    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • 組織特異性
    Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • 配列類似性
    Belongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 発生段階
    Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • ドメイン
    The POU-specific domain mediates interaction with PKM2.
  • 翻訳後修飾
    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • 細胞内局在
    Nucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Information by UniProt

画像

  • SDS-PAGE analysis of 5µg ab95845
  • Indirect ELISA detection of immobilized ab95845.

参考文献

ab95845 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting Abcam.

I have identified a pair of antibodies that have been tested and guaranteed to work in sandwich ELISA against the human Oct4 protein, a protein involved in stem cell self-renewal. Links to the antibodies are giv...

Read More

This Oct3/4 is Isoform 1; the cDNA clone used to produce it is Transcript Variant 1- mRNA Reference Sequence NM_002701.3, http://www.ncbi.nlm.nih.gov/nuccore/54112126

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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