Recombinant Human Notch2 protein (ab114827)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Wheat germ
  • アミノ酸配列
    • アクセッション番号Q04721
    • 生物種Human
    • 配列QCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNG GTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSR DTYECTCQVG
    • 分子量38 kDa including tags
    • 領域27 to 136

特性

Our Abpromise guarantee covers the use of ab114827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    ELISA

    SDS-PAGE

    Western blot

  • 製品の状態Liquid
  • 備考Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

関連情報

  • 別名
    • AGS2
    • hN2
    • Motch B
    • N2
    • N2ECD
    • N2ICD
    • neurogenic locus notch homolog protein 2
    • NOTC2_HUMAN
    • Notch 2
    • Notch 2 intracellular domain
    • Notch Drosophila homolog 2
    • Notch homolog 2
    • Notch homolog 2 Drosophila
    • Notch2
    see all
  • 機能Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
  • 組織特異性Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
  • 関連疾患Alagille syndrome 2
    Hajdu-Cheney syndrome
  • 配列類似性Belongs to the NOTCH family.
    Contains 6 ANK repeats.
    Contains 35 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • 翻訳後修飾Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Hydroxylated by HIF1AN.
    Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.
  • 細胞内局在Cell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737).
  • Information by UniProt

Recombinant Human Notch2 protein 画像

  • 12.5% SDS-PAGE Stained with Coomassie Blue

Recombinant Human Notch2 protein (ab114827) 使用論文

ab114827 has not yet been referenced specifically in any publications.

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