Recombinant Human Neuroserpin protein (ab63224)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL

特性

Our Abpromise guarantee covers the use of ab63224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

    HPLC

    Western blot

  • エンドトキシン・レベル< 0.100 Eu/µg
  • 精製度> 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

関連情報

  • 別名
    • DKFZp781N13156
    • Neuroserpin
    • NEUS_HUMAN
    • Peptidase inhibitor 12
    • PI-12
    • PI12
    • Protease inhibitor 12
    • Serine or cysteine proteinase inhibitor clade I (neuroserpin) member 1
    • Serine or cysteine proteinase inhibitor clade I member 1
    • Serpin I1
    • Serpin peptidase inhibitor clade I (neuroserpin) member 1
    • SERPINI1
    see all
  • 機能Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.
  • 組織特異性Predominantly expressed in the brain.
  • 関連疾患Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B) [MIM:604218]. FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin.
  • 配列類似性Belongs to the serpin family.
  • 細胞内局在Secreted.
  • Information by UniProt

Recombinant Human Neuroserpin protein 画像

  • Anti-Neuroserpin antibody (ab16171) at 1/2500 dilution + Recombinant Human Neuroserpin protein (ab63224) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Recombinant Human Neuroserpin protein (ab63224) 使用論文

This product has been referenced in:
  • Subhadra B  et al. Neuroserpin up-regulation in the Alzheimer's disease brain is associated with elevated thyroid hormone receptor-ß1 and HuD expression. Neurochem Int 63:476-81 (2013). Read more (PubMed: 24036060) »

See 1 Publication for this product

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Application Other

Review text: Comparing the tPA inhibitory activity of Abcam Neuroserpin versus mouse neuroserpin

Procedure: tPA proteolytic assay using

Results:
Mouse neuroserpin inhibited ~70% tPA activity in 5 minutes where as Abcam neuroserpin inhibited ~20 % of tPA activity in 5 minutes. A 1:100 molar ratio of
Abcam NS to tPA gave no better inhibition, if anything it was less inhibitory.
Sample: Escherichia coli

Primary antibody (in addition to 'Neuroserpin protein')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used
Username

Abcam user community

Verified customer

投稿 Mar 07 2012

DISCOUNT CODE: ************ Expiration date:************* I am very pleased to hear you would like to use ab63224 in an Inhibition Assayand send us an image of your results. This code will give you 1 free PROTEIN before the expiration date. To redeem t...

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Thank you for contacting Abcam. We have not tested ab63224 to see if it inhibits TPA activity. We do not have sample sizes of our products, but what I can offer is out testing disount program. Asab63224 has not been tested in an inhibition assay, I can...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"