Recombinant Human Neuroglobin protein (ab63278)

製品の概要

  • 製品名Recombinant Human Neuroglobin protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列M-ERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSFSTVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDG E

関連製品

特性

Our Abpromise guarantee covers the use of ab63278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    ELISA

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.
    Please filter the product by an appropriate sterile filter before using it in the cell culture.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M PBS, pH 7.2

関連情報

  • 別名
    • Neuroglobin
    • NGB
    • NGB_HUMAN
  • 機能Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
  • 組織特異性Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus.
  • 配列類似性Belongs to the globin family.
  • 翻訳後修飾A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO.
    Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity.
  • 細胞内局在Perikaryon. Cytoplasm. Mitochondrion.
  • Information by UniProt

Recombinant Human Neuroglobin protein 画像

  • LANE 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa. LANE 2. reduced and heated sample, 5µg/lane. LANE 3. non-reduced and non-heated sample, 5µg/lane.
  • Anti-Neuroglobin antibody [13C8] (ab37258) at 1 µg/ml + Recombinant Human Neuroglobin protein (ab63278) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

Recombinant Human Neuroglobin protein (ab63278) 使用論文

ab63278 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"