製品の概要

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      115 kDa including tags
    • 領域
      1 to 979
    • タグ
      GST tag N-Terminus
    • 配列の追加情報
      1-979; deleted fragment of 346-731

関連製品

特性

Our Abpromise guarantee covers the use of ab125614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    The specific activity of ab125614 was determined to be 120 nmol/mg/min.
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度
    > 75 % SDS-PAGE.
    Purity determined to be >75% by densitometry.
  • 製品の状態
    Liquid
  • 備考

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.004% EGTA, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • KIAA1995
    • APUG
    • DKFZp434D0935
    • LCCS10
    • MGC138306
    • MGC16714
    • NC
    • NEK 9
    • Nek8
    • nek9
    • NEK9_HUMAN
    • NERCC
    • Nercc 1 kinase
    • NERCC1
    • Nercc1 kinase
    • Never in mitosis A related kinase 9
    • Never in mitosis A-related kinase 9
    • NIMA (never in mitosis gene a) related kinase 9
    • NIMA related kinase 8
    • NIMA related kinase 9
    • NIMA related kinase Nek8
    • NimA related protein kinase 9
    • NIMA-related kinase 8
    • NimA-related protein kinase 9
    • Serine/threonine protein kinase Nek9
    • Serine/threonine-protein kinase Nek9
    see all
  • 機能
    Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.
  • 組織特異性
    Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.
  • 配列類似性
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
    Contains 6 RCC1 repeats.
  • 発生段階
    Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.
  • ドメイン
    Dimerizes through its coiled-coil domain.
  • 翻訳後修飾
    Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt

画像

  • Sample Kinase Assay showing the specific activity of ab125614 as 120 nmol/min/mg.
  • SDS-PAGE analysis of ab125614.

参考文献

ab125614 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab125614.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録