Recombinant human NEK2 protein (ab42599)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: Inhibition Assay
製品の詳細
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製品名
Recombinant human NEK2 protein -
生理活性
100 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.5 mM ATP, 0.3 mg/ml MBPsubstrate, and 0.2 ug/ml NEK2.
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精製度
> 70 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab42599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Inhibition Assay
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製品の状態
Liquid -
備考
Expressed in a Baculovirus infected Sf9 cell expression system. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 40% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- HSPK 21
- HsPK21
- NEK 2
see all -
機能
Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not. -
組織特異性
Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. -
配列類似性
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
発生段階
Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase. -
翻訳後修飾
It is unsure whether Thr-170 or Ser-171 is phosphorylated. -
細胞内局在
Cytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab42599 は論文での使用が確認できていません。