Recombinant human Myosin light chain kinase protein (ab55674)




  • 由来Recombinant
  • 由来Insect cells
  • アミノ酸配列
    • 生物種Human
    • 領域1425 to 1776



Our Abpromise guarantee covers the use of ab55674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    Specific Activity: 200nm/min/mg.

  • アプリケーション


    Functional Studies

  • 製品の状態Liquid
  • 備考

    ab60014 (Mouse MYL9 full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...


  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.


  • 別名
    • deglutamylated form
    • DKFZp686I10125
    • EC
    • FLJ12216
    • Kinase related protein
    • Kinase-related protein
    • KRP
    • MLCK
    • MLCK1
    • MLCK108
    • MLCK210
    • MSTP083
    • MYLK
    • MYLK1
    • Myosin light chain kinase
    • Myosin light polypeptide kinase
    • OTTHUMP00000180642
    • OTTHUMP00000180643
    • smMLCK
    • smooth muscle
    • Smooth muscle myosin light chain kinase
    • Telokin
    see all
  • 機能Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
  • 組織特異性Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 fibronectin type-III domain.
    Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻訳後修飾MLCK is probably down-regulated by phosphorylation.
    The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
  • Information by UniProt

Recombinant human Myosin light chain kinase protein 画像

Recombinant human Myosin light chain kinase protein (ab55674) 使用論文

ab55674 has not yet been referenced specifically in any publications.

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The molecular weight given on website includes molecular weight of tags.
Please find attached the data regarding these two products.
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Thank you for contacting us.
The proteins will run at ˜72kDa, ab55674 and ˜17kDa, ab94519.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submi...

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Thank you for contacting us. Regarding the enquiry for MLCK, you do require the Ca/Calmodulin Solution to run the kinase assay. Please let me know if you have any further questions.