Recombinant Human Myelin Basic Protein (ab43614)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P02686-3
    • 生物種Human
    • 配列MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFM ASQKRPSQRHGSKYLATAST MDHARHGFLP RHRDTGILDS IGRFFGGDRG APKRGSGKDS HHPARTAHYGSLPQKSHGRT QDENPVVHFF KNIVTPRTPP PSQGKGRGLS LSRFSWGAEG QRPGFGYGGRASDYKSAHKG FKGVDAQGTL SKIFKLGGRD SRSGSPMARR
    • タグGST tag N-Terminus
    • 配列の追加情報GST-tag ID: P08515 (226aa), Thrombin site: 221-226aa, 3-AA Linker: 227-229aa, MBP protein: 230-400aa

特性

Our Abpromise guarantee covers the use of ab43614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

  • 製品の状態Liquid
  • 備考

    ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for the following active protein Kinases:

     

    ab51435 (Active human Aurora B full length protein)
    ab55692 (Active human CaMKK full length protein)
    ab55700 (Active human MAP3K8 protein fragment)

    ab55712 (Active human MAP4K6 protein fragment)

    ab55721 (Active Raf1 (mutated F341 Q) protein fragment)
    ab60013 (Active human MEK2 full length protein)

    ab60300 (Active ITK protein fragment)
    ab60325 (Active human ASK1 protein fragment)
    ab60341 (Active human MST4 full length protein)
    ab60900 (Active TXK protein fragment)
    ab61414 (Active mouse PAK3 full length protein)
    ab63173 (Active TGF beta Receptor II protein fragment)
    ab63177 (Active human IPL-1/STK13/Aurora C full length protein)

     

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

関連情報

  • 別名
    • GDB
    • Golli MBP
    • Golli MBP; myelin basic protein
    • Hemopoietic MBP
    • HMBPR
    • HUGO
    • MBP
    • MBP_CAVPO
    • MBP_HUMAN
    • MGC99675
    • MLD
    • Myelin A1 protein
    • Myelin A1 Protein, basic
    • Myelin basic protein
    • Myelin Deficient
    • Myelin membrane encephalitogenic protein
    • OTTHUMP00000163776
    • OTTHUMP00000174387
    • OTTHUMP00000174388
    • SHI
    • Shiverer
    • SP
    see all
  • 機能The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • 組織特異性MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • 関連疾患Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • 配列類似性Belongs to the myelin basic protein family.
  • 発生段階Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • 翻訳後修飾Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • 細胞内局在Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt

Recombinant Human Myelin Basic Protein (ab43614) 使用論文

ab43614 has not yet been referenced specifically in any publications.

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