製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Wheat germ
  • アミノ酸配列
    • アクセッション番号P42345
    • 生物種Human
    • 配列WGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDL LDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWW
    • 分子量37 kDa including tags
    • 領域1521 to 1620

特性

Our Abpromise guarantee covers the use of ab114179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    SDS-PAGE

    ELISA

  • 製品の状態Liquid
  • 備考Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

関連情報

  • 別名
    • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1)
    • FK506 binding protein 12 rapamycin associated protein 1
    • FK506 binding protein 12 rapamycin associated protein 2
    • FK506 binding protein 12 rapamycin complex associated protein 1
    • FK506-binding protein 12-rapamycin complex-associated protein 1
    • FKBP rapamycin associated protein
    • FKBP12 rapamycin complex associated protein
    • FKBP12-rapamycin complex-associated protein
    • FKBP12-rapamycin complex-associated protein 1
    • FLJ44809
    • FRAP
    • FRAP1
    • FRAP2
    • Mammalian target of rapamycin
    • Mechanistic target of rapamycin
    • mTOR
    • MTOR_HUMAN
    • OTTHUMP00000001983
    • RAFT1
    • Rapamycin and FKBP12 target 1
    • Rapamycin associated protein FRAP2
    • Rapamycin target protein
    • Rapamycin target protein 1
    • RAPT1
    • Serine/threonine-protein kinase mTOR
    see all
  • 機能Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
  • 組織特異性Expressed in numerous tissues, with highest levels in testis.
  • 配列類似性Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 7 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • 翻訳後修飾Autophosphorylated; when part of mTORC1 or mTORC2.
  • 細胞内局在Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
  • Information by UniProt

Recombinant Human mTOR protein 画像

  • ab114179 on a 12.5% SDS-PAGE Stained with Coomassie Blue.

Recombinant Human mTOR protein (ab114179) 使用論文

ab114179 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"