Recombinant human TTK/Mps1 protein (ab89589)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB, Functional Studies
製品の詳細
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製品名
Recombinant human TTK/Mps1 protein -
生理活性
The Specific activity of ab89589 was determined to be 19 nmol/min/mg.
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精製度
> 75 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
タグ
GST tag N-Terminus
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関連製品
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Related Products
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab89589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
Functional Studies
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製品の状態
Liquid -
備考
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- cancer/testis antigen 96
- CT96
- Dual specificity protein kinase TTK
see all -
機能
Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint. -
組織特異性
Present in rapidly proliferating cell lines. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. - Information by UniProt
画像
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The specific activity of TTK/Mps1 (ab89589) was determined to be 18 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab89589
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SDS PAGE analysis of ab89589
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SDS-PAGE showing ab89589 at approximately 130kDa.
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Kinase Assay demonstrating specific activity of ab89589.
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Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 10 µg/ml +
Recombinant human TTK/Mps1 protein (ab89589) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 8 minutesab11108 recognizes the full length tagged recombinant TTK/Mps1 protein (ab89589) which has an expected molecular weight of 130 kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab89589 は論文での使用が確認できていません。