製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 配列
      MRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PETPLVPTEP VPPEPGTPAN CDPALSFDAV STLRGEILIF KDRHFWRKSL RKLEPELHLI SSFWPSLPSG VDAAYEVTSK DLVFIFKGNQ FWAIRGNEVR AGYPRGIHTL GFPPTVRKID AAISDKEKNK TYFFVEDKYW RFDEKRNSME PGFPKQIAED FPGIDSKIDA VFEEFGFFYF FTGSSQLEFD PNAKKVTHTL KSNSWLNC
    • 分子量
      43 kDa
    • 領域
      101 to 477

特性

Our Abpromise guarantee covers the use of ab96555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    ab96555 activity was measured by its ability to cleave a chromogenic peptide MMP3 substrate at room temperature. At a MMP3 concentration of 2.5 µg/ml, 50% cleavage was achieved at an incubation time of approximately 75 minutes.
  • アプリケーション

    SDS-PAGE

    Functional Studies

    Western blot

  • エンドトキシン・レベル
    < 0.100 Eu/µg
  • 精製度
    > 95 % SDS-PAGE.
    Purity is greater than 98% by SDS-PAGE gel and HPLC analyses.
  • 製品の状態
    Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.00111% Calcium chloride, 0.164% Sodium phosphate, 0.1% CHAPS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.

関連情報

  • 別名
    • CHDS6
    • Matrix metalloproteinase 3
    • Matrix metalloproteinase-3
    • MGC126102
    • MGC126103
    • MGC126104
    • MMP 3
    • MMP-3
    • MMP3
    • MMP3_HUMAN
    • Proteoglycanase
    • SL-1
    • SL1
    • STMY
    • STMY1
    • STR1
    • Stromelysin 1
    • Stromelysin-1
    • Transin 1
    • Transin-1
    see all
  • 機能
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 配列類似性
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメイン
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

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  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

参考文献

This product has been referenced in:
  • O'Callaghan J  et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). Read more (PubMed: 28158775) »

See 1 Publication for this product

レビューと Q&A

The protocol for recombinant human MMP-3/MMP-2 bioactivity used is as follows:

Kit used is supplied by AnaSpec (Catolog#72095)


1. Dilute supplied colorimetric substrate 1:50 in assay buffer

2. Reconsitute MMP-3/MMP-2 sam...

Read More

We use the kit below to test both of these MMP proteins. All assays are run using the instructions in the kit, as well as the buffers they supply.

http://www.anaspec.com/products/product.asp?id=44834

Thank you for contacting us.

To our knowledge, this MMP3 protein has not been tested in a mouse system.

The amino acid sequence of the protein has approximately 78% identity to Mouse MMP3. Though not identical, it may still be cap...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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