Recombinant human MMP1 protein (ab124850)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • アクセッション番号P03956
    • 生物種Human
    • 分子量52 kDa including tags
    • 領域1 to 469
    • タグHis tag C-Terminus
    • 配列の追加情報MMP-1 Human Recombinant produced in HEK293 cells is a proform of the Human MMP1 (Met1-Asn469) and fused with a polyhistide tag at the C-terminus.

特性

Our Abpromise guarantee covers the use of ab124850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The activity was measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2. The specific activity is > 400 pmoles/min/µg.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 95 % SDS-PAGE.
    ab124850 is purified by proprietary chromatographic techniques and 0.2µm sterile filtered.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Constituents: 25% MES, 25% Brij, 25% Glycerol, 25% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • 27 kDa interstitial collagenase
    • CLG
    • CLGN
    • collagenase, fibroblast
    • collagenase, interstitial
    • Fibroblast collagenase
    • Interstitial collagenase
    • Matrix metallopeptidase 1 (interstitial collagenase)
    • Matrix metalloprotease 1
    • Matrix Metalloproteinase 1
    • Matrix metalloproteinase-1
    • MMP 1
    • MMP-1
    • MMP1
    • MMP1_HUMAN
    • OTTHUMP00000045866
    see all
  • 機能Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメインThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

Recombinant human MMP1 protein (ab124850) 使用論文

ab124850 has not yet been referenced specifically in any publications.

Product Wall



It is provided as an active protein.

Thank you for enquiry.

The concentration of our batch number currently available is 0.43 mg/ml.

I hope this helps and if we can assist further, please do not hesitate to contact us.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"