Recombinant human MIF protein (ab75432)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 配列MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

特性

Our Abpromise guarantee covers the use of ab75432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 95 % SDS-PAGE.
    Purity is approximately 97% as determined by analysis by RP-HPLC and reducing and non-reducing SDS-PAGE. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
  • 製品の状態Lyophilised
  • 備考


    ab75432 acts as an inflammatory mediator consisting of two alpha-helices and six beta-strands, four of which form a beta-sheet structure. The remaining two beta-strands interact with other MIF molecules forming a trimer in solution. MIF acts on fibroblasts by inducing IL-1, IL-8 and MMP expression. MIF stimulates NO production and TNF-alpha release following IFN-gamma activation on macrophages.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10mM Sodium phosphate, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Reconstitute with sterile water at 0.1 - 1.0 mg/ml, which can then be further diluted to other aqueous solutions.

関連情報

  • 別名
    • acrophage migration inhibitory factor (glycosylation-inhibiting factor)
    • GIF
    • GLIF
    • Glycosylation inhibiting factor
    • Glycosylation-inhibiting factor
    • L-dopachrome isomerase
    • L-dopachrome tautomerase
    • Macrophage migration inhibitory factor
    • MIF
    • MIF protein
    • MIF_HUMAN
    • MMIF
    • Phenylpyruvate tautomerase
    see all
  • 機能Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 関連疾患Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  • 配列類似性Belongs to the MIF family.
  • 細胞内局在Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt

Recombinant human MIF protein 画像

  • ab75432 used in Western Blot. Figure: 1 ug run under (+) reducing and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human MIF has a predicted MW of 12.5 kDa.

Recombinant human MIF protein (ab75432) 使用論文

ab75432 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"