Recombinant human Met (c-Met) (mutated M1250 T) protein (ab119729)

製品の概要

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      81 kDa including tags
    • 領域
      956 to 1390

特性

Our Abpromise guarantee covers the use of ab119729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Specific activity of ab119729 was determined to be 185 nmol/min/mg as per activity assay protocol.
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度
    > 85 % Proprietary Purification.
    determined by densitometry
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.79% Tris buffered saline, 0.88% Sodium chloride, 0.003% EDTA, 0.003% DTT, 0.002% PMSF, 25% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • AUTS9
    • c met
    • D249
    • Hepatocyte growth factor receptor
    • HGF
    • HGF receptor
    • HGF/SF receptor
    • HGFR
    • MET
    • Met proto oncogene tyrosine kinase
    • MET proto oncogene, receptor tyrosine kinase
    • Met proto-oncogene
    • Met proto-oncogene (hepatocyte growth factor receptor)
    • Met protooncogene
    • MET_HUMAN
    • Oncogene MET
    • Par4
    • Proto-oncogene c-Met
    • RCCP2
    • Scatter factor receptor
    • SF receptor
    • Tyrosine-protein kinase Met
    see all
  • 機能
    Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.
  • 関連疾患
    Note=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
    Note=Defects in MET may be associated with gastric cancer.
    Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
    Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
    Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
    Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 3 IPT/TIG domains.
    Contains 1 protein kinase domain.
    Contains 1 Sema domain.
  • ドメイン
    The kinase domain is involved in SPSB1 binding.
  • 翻訳後修飾
    Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365.
  • 細胞内局在
    Membrane.
  • Information by UniProt

画像

  • The specific activity of ab119729 was determined to be 185 nmol /min/mg as per activity assay protocol.
  • The purity of ab119729 was determined to be >85% by densitometry, approx. MW 81 kDa.

参考文献

ab119729 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab119729.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録