製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号Q14680
    • 生物種Human
    • 分子量61 kDa including tags
    • 領域1 to 340

関連製品

特性

Our Abpromise guarantee covers the use of ab102551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The Specific activity of ab102551 was determined to be 200 nmol/min/mg.
  • アプリケーション

    Functional Studies

    SDS-PAGE

    Western blot

  • 精製度> 85 % Densitometry.
    Purity was determined to be >85% by densitometry.
  • 製品の状態Liquid
  • 備考

    ab204885 (ZIP Kinase peptide substrate) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • AI327312
    • hMELK
    • HPK 38
    • hPK38
    • KIAA0175
    • Likely ortholog of maternal embryonic leucine zipper kinase
    • Maternal embryonic leucine zipper kinase
    • MELK
    • MELK_HUMAN
    • mKIAA0175
    • MPK38
    • OTTHUMP00000021377
    • OTTHUMP00000046113
    • pEg3 kinase
    • Protein kinase Eg3
    • Protein kinase PK38
    • RP23 382O11.1
    • Tyrosine protein kinase MELK
    see all
  • 機能Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
  • 組織特異性Expressed in placenta, kidney, thymus, testis, ovary and intestine.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
  • 細胞内局在Cytoplasm.
  • Information by UniProt

Recombinant human MELK protein 画像

  • The Specific activity of ab102551 was determined to be 200 nmol/min/mg.
  • SDS-PAGE showing ab102551 at approximately 61kDa.

Recombinant human MELK protein (ab102551) 使用論文

ab102551 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab102551.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"