Recombinant human MELK protein (ab102551)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
製品の詳細
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製品名
Recombinant human MELK protein
MELK タンパク質・ペプチド 製品一覧 -
生理活性
The Specific activity of ab102551 was determined to be 200 nmol/min/mg.
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精製度
> 85 % Densitometry.
Purity was determined to be >85% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
61 kDa including tags -
領域
1 to 340
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特性
Our Abpromise guarantee covers the use of ab102551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
Western blot
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製品の状態
Liquid -
備考
ab204885 (ZIP Kinase peptide substrate) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- AI327312
- hMELK
- HPK 38
see all -
機能
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis. -
組織特異性
Expressed in placenta, kidney, thymus, testis, ovary and intestine. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8. -
細胞内局在
Cytoplasm. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab102551 は論文での使用が確認できていません。