Recombinant human MCSF Receptor protein (Fc Chimera) (ab83682)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • アクセッション番号P07333
    • 生物種Human
    • 配列Theoretical Sequence: IPVIEPSVPELVVKPGATVTLRCVGNGSVEWDGPPSPHWTLYSDGSSSIL STNNATFQNTGTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAQEVVVFE DQDALLPCLLTDPVLEAGVSLVRVRGRPLMRHTNYSFSPWHGFTIHRAKF IQSQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPAELVRIRGEA AQIVCSASSVDVNFDVFLQHNNTKLAIPQQSDFHNNRYQKVLTLNLDQVD FQHAGNYSCVASNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGL NLKVMVEAYPGLQGFNWTYLGPFSDHQPEPKLANATTKDTYRHTFTLSLP RLKPSEAGRYSFLARNPGGWRALTFELTLRYPPEVSVIWTFINGSGTLLC AASGYPQPNVTWLQCSGHTDRCDEAQVLQVWDDPYPEVLSQEPFHKVTVQ SLLTVETLEHNQTYECRAHNSVGSGSWAFIPISAGAHTHPPDEGSSNTKV DKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTC VVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQ DWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKN QVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLT VDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    • 領域1 to 512
    • 配列の追加情報Fused with the Fc region of Human IgG1 at the C-terminus.

特性

Our Abpromise guarantee covers the use of ab83682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Activity: The ED50 of ab83682 is typically 0.2 - 0.3 ug/ml as measured by its ability to neutralize MCSF mediated proliferation of mNFS-60 cells.
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

関連情報

  • 別名
    • C FMS
    • CD 115
    • CD115
    • CD115 antigen
    • CFMS
    • Colony stimulating factor 1 receptor
    • Colony stimulating factor I receptor
    • CSF 1 R
    • CSF 1R
    • CSF-1 receptor
    • CSF-1-R
    • CSF1 R
    • CSF1R
    • CSF1R_HUMAN
    • CSFR
    • EC 2.7.10.1
    • FIM 2
    • FIM2
    • FMS
    • FMS proto oncogene
    • FMS protooncogene
    • HDLS
    • M-CSF Receptor
    • M-CSF-R
    • Macrophage colony stimulating factor 1 receptor
    • Macrophage colony stimulating factor I receptor
    • Macrophage colony-stimulating factor 1 receptor
    • McDonough feline sarcoma viral (v fms) oncogene homolog
    • MCSFR
    • Oncogen FMS
    • Proto-oncogene c-Fms
    • V-FMS McDonough feline sarcoma viral oncogen homolog, formerly
    see all
  • 機能Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
  • 組織特異性Expressed in bone marrow and in differentiated blood mononuclear cells.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Membrane.
  • Information by UniProt

Recombinant human MCSF Receptor protein (Fc Chimera) 画像

  • Densitometry of protein isoforms visualised by 2-DE. The triangle indicates the theoretical MW and pI of the protein.
  • 1D SDS-PAGE of ab83682 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1 MW markers; Lane 2 ab83682; Lane 3 ab83682 treated with PNGase F to remove potential N-linked glycans; Lane 4 ab83682 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane.

    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.

  • A sample of ab83682 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Approximately 40 µg of protein was load; Gel was stained using Deep Purple™.

Recombinant human MCSF Receptor protein (Fc Chimera) (ab83682) 使用論文

ab83682 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting me.

I received an answer from the lab. The lab confirmed, that for the ab83682 a NuPage 4-20% Tris-Glycine SDS gel was used together with a standard buffer system.

The lab also confirmed, that it is not recomm...

Read More

Thank you very much for providing your WB result.

1. As far as I can see the band detected in samples 5 to 7 are at the correct size between 100-140 kDa. I think the band that shows up at about 70 kda might still be the BSA. Because of the hig...

Read More

Thank you for this new image.

It would be beneficial to have a new western blot result to assure which bands are the MCSF protein. Like mentioned before, I suspect the strong band at about 77 kDa might be BSA, which is a content of the storage...

Read More

Thank you for your email.

Thank you for giving me this further information. I am pleased that you will try the given suggestions for the experiment. I hope this might be helpful for you.

I can confirm that I think there are bands at t...

Read More

I apologize for making you wait this long. Regrettably I did not receive a confirmation about the used gel from the lab. I wrote them a reminder email as you did with me. Thank you very much for this.

Meanwhile I was looking through the data y...

Read More

I like to reassure you that we have not forgotten about your inquiry. Anja is currently out of office. However, I know about your inquiry and I will get back to you as soon as I receive an answer from the lab. Regrettably I have not received the needed...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"