製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 配列
      MGSSHHHHHH SSGLVPRGSH MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE ECQQDVSLLR KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY IYSSGSVEAQ KLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLIT SFSELYLPSS T
    • 分子量
      31 kDa including tags
    • 領域
      1 to 261
    • タグ
      His tag N-Terminus
    • 配列の追加情報
      ENOPH1 Human Recombinant fused with a20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing281 amino acids (1-261 a.a.).

特性

Our Abpromise guarantee covers the use of ab125778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度
    > 90 % SDS-PAGE.
    ab125778 was purified by proprietary chromatographic techniques and filter sterilized.
  • 製品の状態
    Liquid
  • 備考
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at -20°C. Store under desiccating conditions.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

関連情報

  • 別名
    • 2
    • 2 3 diketo 5 methylthio 1 phosphopentane phosphatase
    • 3-diketo-5-methylthio-1-phosphopentane phosphatase
    • Acireductone synthase
    • DKFZp586M0524
    • E 1 enzyme
    • E1
    • Enolase phosphatase 1
    • Enolase phosphatase E1
    • Enolase-phosphatase E1
    • ENOPH_HUMAN
    • Enoph1
    • FLJ12594
    • MASA homolog
    • MST145
    • MSTP145 protein
    see all
  • 機能
    Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
  • パスウェイ
    Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.
    Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
  • 配列類似性
    Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant Human MASA protein (ab125778) 使用論文

ab125778 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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