製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号Q96L34
    • 生物種Human
    • 分子量104 kDa including tags
    • 領域1 to 752

関連製品

特性

Our Abpromise guarantee covers the use of ab105211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The Specific activity of ab105211 was determined to be 1050 nmol/min/mg.
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度> 90 % SDS-PAGE.
    Purity was determined to be >90% by densitometry.
  • 製品の状態Liquid
  • 備考

    ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • FLJ90097
    • KIAA1860
    • MAP / microtubule affinity regulating kinase 4
    • MAP / microtubule affinity regulating kinase 4L
    • MAP/microtubule affinity regulating kinase 4
    • MAP/microtubule affinity regulating kinase like 1
    • MAP/microtubule affinity-regulating kinase 4
    • MAP/microtubule affinity-regulating kinase-like 1
    • MARK 4
    • MARK 4L
    • MARK L1
    • MARK4
    • MARK4 serine / threonine protein kinase
    • MARK4 serine/threonine protein kinase
    • MARK4_HUMAN
    • MARK4S
    • MARKL 1
    • MARKL1
    • Nbla00650
    • Putative protein product of Nbla00650
    see all
  • 機能Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942). Phosphorylates the microtubule-associated protein MAPT (PubMed:14594945, PubMed:23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532). Reduces neuronal cell survival (PubMed:15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942).
  • 組織特異性Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • 発生段階Expressed at all stages of the mitotic cell cycle.
  • 翻訳後修飾Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
    Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).
  • 細胞内局在Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.
  • Information by UniProt

Recombinant human MARK4 protein 画像

  • SDS-PAGE showing ab105211 at approximately 104kDa.
  • The Specific activity of ab105211 was determined to be 1050 nmol/min/mg.

Recombinant human MARK4 protein (ab105211) 使用論文

ab105211 has not yet been referenced specifically in any publications.

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