Recombinant human Mark3 protein (ab60012)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Mark3 protein -
生理活性
Specific activity 722 nmol/min/mg.
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精製度
> 75 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Related Products
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab60012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- C-TAK1
- Cdc25C associated protein kinase 1
- Cdc25C-associated protein kinase 1
see all -
機能
Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. - Information by UniProt
画像
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The specific activity of Mark3 (ab60012) was determined to be 293.5 nmol/min/mg as per activity assay protocol and was equivalent to 830 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab60012
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Sample Kinase Activity Plot.
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ab60012 on SDS-PAGE. The extra bands are thought to be truncated MARK3 and tag.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab60012 は論文での使用が確認できていません。