Recombinant human MARK2 protein (ab119135)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
製品の詳細
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製品名
Recombinant human MARK2 protein -
生理活性
Specific Activity: 725 - 795 nmol/min/mg
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精製度
> 70 % SDS-PAGE.
Purity was determined to be >70% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
114 kDa including tags -
領域
1 to 788
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab119135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
Functional Studies
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製品の状態
Liquid -
備考
ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- ELKL motif kinase
- ELKL motif kinase 1
- EMK-1
see all -
機能
Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. -
組織特異性
High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
細胞内局在
Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane. - Information by UniProt
画像
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The specific activity of MARK2 (ab119135) was determined to be 790 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab119135
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SDS PAGE analysis of ab119135
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Sample Kinase Activity Plot: The specific activity of ab119135 was determined to be 795 nmol/min/mg.
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SDS-PAGE analysis of ab119135.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab119135 は論文での使用が確認できていません。