製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Wheat germ
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 配列
      LEERFSDDQLVSMSVRELNRQLRGFSKEEVIRLKQKRRTLKNRGYAQSRF KRVQQRHILESEKCQLQSQVEQLKLEVGRLAKERDL
    • 分子量
      35 kDa including tags
    • 領域
      222 to 308

特性

Our Abpromise guarantee covers the use of ab112437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    ELISA

    Western blot

  • 製品の状態
    Liquid
  • 備考
    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.31% Glutathione

関連情報

  • 別名
    • HMafA
    • Mafa
    • MAFA_HUMAN
    • Pancreatic beta cell specific transcriptional activator
    • Pancreatic beta-cell-specific transcriptional activator
    • RIPE3b1
    • Transcription factor MafA
    • Transcription factor RIPE3b1
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog A
    • v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)
    • V-maf musculoaponeurotic fibrosarcoma oncogene homolog A
    see all
  • 機能
    Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
  • 配列類似性
    Belongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • 翻訳後修飾
    Ubiquitinated, leading to its degradation by the proteasome.
    Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
  • 細胞内局在
    Nucleus. Detected in nuclei of pancreas islet beta cells.
  • Information by UniProt

Recombinant Human MafA protein 画像

  • 12.5% SDS-PAGE showing ab112437 at approximately 35.2kDa stained with Coomassie Blue.

Recombinant Human MafA protein (ab112437) 使用論文

ab112437 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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