Recombinant Human Lysyl tRNA synthetase protein (ab113142)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号Q15046
    • 生物種Human
    • 配列MGSSHHHHHHSSGLVPRGSHMGSGVGPEEESVDPNQYYKIRSQAIHQLKV NGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASG GKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPG KTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDF VRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNE LDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCE FYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTP PFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRT TARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELF VMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEY GLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLE STTVGTSV
    • 分子量64 kDa including tags
    • 領域63 to 597
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab113142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.
    ab113142 was purified using conventional chromatography.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride, 0.02% DTT

関連情報

  • 別名
    • CMTRIB
    • DFNB89
    • EC 6.1.1.6
    • KARS
    • KARS 1
    • KARS 2
    • KARS1
    • KARS2
    • KIAA0070
    • KRS
    • Lysine tRNA ligase
    • Lysine--tRNA ligase
    • LysRS
    • Lysyl-tRNA synthetase
    • SYK_HUMAN
    see all
  • 機能Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
  • 関連疾患Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB) [MIM:613641]; also called Charcot-Marie-Tooth neuropathy recessive intermediate B. CMTRIB is a form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Recessive intermediate forms of Charcot-Marie-Tooth disease are characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • 配列類似性Belongs to the class-II aminoacyl-tRNA synthetase family.
  • ドメインThe N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain (By similarity), is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2.
  • 細胞内局在Mitochondrion and Cytoplasm. Nucleus. Cell membrane. Secreted. Secretion is induced by TNF-alpha.
  • Information by UniProt

Recombinant Human Lysyl tRNA synthetase protein 画像

  • 15% SDS-PAGE analysis of ab113142 (3µg)

Recombinant Human Lysyl tRNA synthetase protein (ab113142) 使用論文

ab113142 has not yet been referenced specifically in any publications.

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