Recombinant human Lyn protein (ab70789)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human Lyn protein
Lyn タンパク質・ペプチド 製品一覧 -
生理活性
The Specific activity of ab70789 was determined to be 571 nmol/min/mg.
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精製度
> 90 % SDS-PAGE.
Purity: was determined to be >90% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH DFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMGCI SKGKDSLSD DGVDLKTQPV RNTERTIYVR PTSNKQQRP VPESQLLPGQ RFQTKDPEEQ GDIVVALYPY DGIHPDDLSF KKGEKMKVLE EHGEWWKAKS LTKKEGFIP SNYVAKLNTL ETEEWFFKDI TRKDAERQLL PGNSAGAFL IRESETLKGS FSLSVRDFDP VHGDVIKHYK IRSLDNGGYY ISPRITFPCI SDMIKHYQKQ ADGLCRRLEK ACISPKPQKP WDKDAWEIPR ESIKLVKRLG AGQFGEVWMG YYNNSTKVAV KTLKPGTMSV QAFLEEANLM TLQHDKLVR LYAVVTREEP IYIITEYMAK GSLLDFLKSD EGGKVLLPKL IDFSAQIAEG MAYIERKNYI HRDLRAANVL VSESLMCKIA DFGLARVIED NEYTAREGAK FPIKWTAPEA INFGCFTIKS DVWSFGILLY EIVTYGKIPY PGRTNADVMT LSQGYRMPR VENCPDELYD IMKMCWKEKA ERPTFDYLQ SVLDDFYTAT EGQYQQQP -
予測される分子量
85 kDa -
タグ
GST tag N-Terminus
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関連製品
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Related Products
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab70789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- AA407514
- EC 2.7.10.2
- FLJ26625
see all -
機能
Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents. -
組織特異性
Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
ドメイン
The protein kinase domain plays an important role in its localization in the cell membrane. -
翻訳後修飾
Ubiquitinated. Ubiquitination is SH3-dependent. -
細胞内局在
Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab70789 は論文での使用が確認できていません。