製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 領域42 to 209

特性

Our Abpromise guarantee covers the use of ab89225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度> 90 % SDS-PAGE.
    ab89225 is purified using conventional chromatography techniques.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, pH 8.0

関連情報

  • 別名
    • AL024421
    • CSDD1
    • FLJ12457
    • Lin 28
    • Lin 28 homolog
    • Lin 28 homolog (C. elegans)
    • Lin 28 homolog A
    • Lin 28 homolog A (C. elegans)
    • Lin-28A
    • Lin28
    • Lin28, C. elegans, homolog of, A
    • LIN28A
    • LN28A_HUMAN
    • Protein lin-28 homolog A
    • RNA binding protein lin 28
    • Tex17
    • ZCCHC1
    • Zinc finger CCHC domain containing 1
    • Zinc finger CCHC domain containing protein 1
    • Zinc finger CCHC domain-containing protein 1
    see all
  • 機能Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
  • 組織特異性Expressed in embryonic stem cells (ES cells), placenta and testis.
  • 配列類似性Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • 発生段階Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
  • ドメインThe CSD domain is required for function in muscle differentiation.
  • 細胞内局在Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt

Recombinant Human Lin28 protein 画像

Recombinant Human Lin28 protein (ab89225) 使用論文

ab89225 has not yet been referenced specifically in any publications.

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