製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab60305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Specific activity: 239 nmol/min/mg.
  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態Liquid
  • 備考

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • IMD22
    • LCK
    • Lck p56
    • LCK proto-oncogene, Src family tyrosine kinase
    • LCK_HUMAN
    • Leukocyte C-terminal Src kinase
    • LSK
    • Lymphocyte cell specific protein tyrosine kinase
    • Lymphocyte cell-specific protein-tyrosine kinase
    • Lymphocyte specific protein tyrosine kinase
    • Membrane associated protein tyrosine kinase
    • Oncogene lck
    • P56 LCK
    • p56(LSTRA) protein tyrosine kinase
    • p56-LCK
    • p56lck
    • pp58 lck
    • pp58lck
    • Protein YT16
    • Proto oncogene tyrosine protein kinase LCK
    • Proto-oncogene Lck
    • Protooncogene tyrosine protein kinase LCK
    • T cell specific protein tyrosine kinase
    • T cell-specific protein-tyrosine kinase
    • T lymphocyte specific protein tyrosine kinase p56lck
    • Tyrosine-protein kinase Lck
    • YT 16
    • YT16
    see all
  • 機能Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3.
  • 組織特異性Expressed specifically in lymphoid cells.
  • 関連疾患Note=A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • ドメインThe SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.
  • 翻訳後修飾Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.
  • 細胞内局在Cytoplasm. Cell membrane. Present in lipid rafts in an unactive form.
  • Information by UniProt

Recombinant human Lck protein 画像

  • Sample Kinase assay plot.
  • ab60305 on SDS-PAGE.

Recombinant human Lck protein (ab60305) 使用論文

ab60305 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab60305.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"