Recombinant human KDM6B / JMJD3 protein (ab196420)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 50% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
-
製品名
Recombinant human KDM6B / JMJD3 protein -
生理活性
Specific activity: 0.022 pmole/min/μg.
10µl reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 150 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD3 (100 – 500 ng) added to the wells. Add antibody against demethylated K27 peptide.
-
精製度
>= 50 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
KAKPPTAP APPSAPAPSA QPTPPSASVP GKKAREEAPG PPGVSRADML KLRSLSEGPP KELKIRLIKV ESGDKETFIA SEVEERRLRM ADLTISHCAA DVVRASRNAK VKGKFRESYL SPAQSVKPKI NTEEKLPREK LNPPTPSIYL ESKRDAFSPV LLQFCTDPRN PITVIRGLAG SLRLNLGLFS TKTLVEASGE HTVEVRTQVQ QPSDENWDLT GTRQIWPCES SRSHTTIAKY AQYQASSFQE SLQEEKESED EESEEPDSTT GTPPSSAPDP KNHHIIKFGT NIDLSDAKRW KPQLQELLKL PAFMRVTSTG NMLSHVGHTI LGMNTVQLYM KVPGSRTPGH QENNNFCSVN INIGPGDCEW FAVHEHYWET ISAFCDRHGV DYLTGSWWPI LDDLYASNIP VYRFVQRPGD LVWINAGTVH WVQATGWCNN IAWNVGPLTA YQYQLALERY EWNEVKNVKS IVPMIHVSWN VARTVKISDP DLFKMIKFCL LQSMKHCQVQ RESLVRAGKK IAYQGRVKDE PAYYCNECDV EVFNILFVTS ENGSRNTYLV HCEGCARRRS AGLQGVVVLE QYRTEELAQA YDAFTLVRAR RARGQRRRAL GQAAGTGFGS PAAPFPEPPP AFSPQAPAST SR -
予測される分子量
71 kDa including tags -
領域
1043 to 1643 -
タグ
DDDDK tag C-Terminus -
配列の追加情報
NM_001080424.
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab196420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Functional Studies
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Also contains 90 µg/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- Histone demethylase JMJD3
- JmjC domain containing protein 3
- JmjC domain-containing protein 3
see all -
機能
Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. -
配列類似性
Belongs to the UTX family.
Contains 1 JmjC domain. -
細胞内局在
Nucleus. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab196420 は論文での使用が確認できていません。