Recombinant human KDM5C / Jarid1C / SMCX protein (ab196392)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 85% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human KDM5C / Jarid1C / SMCX protein
KDM5C / Jarid1C / SMCX タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity of 0.078 pmol/min/µg.
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精製度
>= 85 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
EPGSDDFLPPPECPVFEPSWAEFRDPLGYIAKIRPIAEKSGICKIRPPAD WQPPFAVEV DNFRFTPRIQRLNELEAQTRVKLNYLDQIAKFWEIQGSS LKIPNVERRILDLYSLSKIVV EEGGYEAICKDRRWARVAQRLNYPPGK NIGSLLRSHYERIVYPYEMYQSGANLVQCNTRP FDNEEKDKEYKPHSI PLRQSVQPSKFNSYGRRAKRLQPDPEPTEEDIEKNPELKKLQIYG AGP KMMGLGLMAKDKTLRKKDKEGPECPPTVVVKEELGGDVKVESTSPKTFLE SKEELSH SPEPCTKMTMRLRRNHSNAQFIESYVCRMCSRGDEDDKLLL CDGCDDNYHIFCLLPPLPE IPKGVWRCPKCVMAECKRPPEAFGFEQAT REYTLQSFGEMADSFKADYFNMPVHMVPTEL VEKEFWRLVNSIEEDVT VEYGADIHSKEFGSGFPVSDSKRHLTPEEEEYATSGWNLNVMP VLEQS VLCHINADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWY GVPSL AAEHLEEVMKKLTPELFDSQPDLLHQLVTLMNPNTLMSHGVPV VRTNQCAGEFVITFPRA YHSGFNQGYNFAEAVNFCTADWLPAGRQCIE HYRRLRRYCVFSHEELICKMAACPEKLDL NLAAAVHKEMFIMVQEERR LRKALLEKGITEAEREAFELLPDDERQCIKCKTTCFLSALA CYDCPDG LVCLSHINDLCKCSSSRQYLRYRYTLDELPAMLHKLKVRAESFDTWANKV RVA LEVEDGRKRSLEELRALESEARERRFPNSELLQQLKNCLSEAEAC VSRALGLVSGQEAGP HRVAGLQMTLTELRAFLDQMNNLPCAMHQIGDV KGVLEQVEAYQAEAREALASLPSSPGL LQSLLERGRQLGVEVPEAQQL QRQVEQARWLDEVKRTLAPSARRGTLAVMRGLLVAGASV APSPAVDKA QAELQELLTIAERWEEKAHLCLEARQKHPPATLEAIIREAENIPVHLPNI Q ALKEALAKARAWIADVDEIQNGDHYPCLDDLEGLVAVGRDLPVGLEE LRQLELQVLTAHS WREKASKTFLKKNSCYTLLEVLCPCADAGSDSTKR SRWMEKELGLYKSDTELLGLSAQDL RDPGSVIVAFKEGEQKEKEGILQ LRRTNSAKPSPLASSSTASSTTSICVCGQVLAGAGAL QCDLCQDWFHG RCVSVPRLLSSPRPNPTSSPLLAWWEWDTKFLCPLCMRSRRPRLETILA LLVALQRLPVRLPEGEALQCLTERAISWQGRARQALASEDVTALLGRLA ELRQRLQAEPR PEEPPNYPAAPASDPLREGSGKDMPKVQGLLENGDSV TSPEKVAPEEGSGKRDLELLSSL LPQLTGPVLELPEATRAPLEELMME GDLLEVTLDENHSIWQLLQAGQPPDLERIRTLLEL EKAERHGSRARGR ALERRRRRKVDRGGEGDDPAREELEPKRVRSSGPEAEEVQEEEELEE E TGGEGPPAPIPTTGSPSTQENQNGLEPAEGTTSGPSAPFSTLTPRLHLPC PQQPPQQQL -
予測される分子量
181 kDa including tags -
領域
2 to 1560 -
タグ
His tag N-Terminus , DDDDK tag N-Terminus
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関連製品
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Related Products
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
特性
Our Abpromise guarantee covers the use of ab196392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 64% Sodium chloride, 0.02% Potassium chloride, 0.462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
Also contains 80 µg/ml DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- DXS1272E
- Histone demethylase JARID1C
- JARID1C
see all -
機能
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. -
組織特異性
Expressed in all tissues examined. Highest levels found in brain and skeletal muscle. -
関連疾患
Defects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism. -
配列類似性
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers. -
ドメイン
The first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
Both the JmjC domain and the JmjN domain are required for enzymatic activity. -
細胞内局在
Nucleus. - Information by UniProt
画像
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10 µL reaction mix containing assay buffer with 20 mM Tris (pH 7.4), 50 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab196392 (50 – 200 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection.
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10% SDS PAGE with Coomassie staining: 6 µg ab196392.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab196392 は論文での使用が確認できていません。