Recombinant Human KDM1 / LSD1 protein (ab125649)

製品の概要

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      100 kDa including tags
    • 領域
      172 to 852

特性

Our Abpromise guarantee covers the use of ab125649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    SDS-PAGE

  • 精製度
    > 95 % Densitometry.

  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

関連情報

  • 別名
    • Amine oxidase (flavin containing) domain 2
    • Amine oxidase, flavin containing, 2
    • AOF2
    • BHC110
    • BRAF35 HDAC complex protein BHC110
    • BRAF35-HDAC complex protein BHC110
    • BRAF35/HDAC complex, 110-kD subunit
    • CPRF
    • EC1
    • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit
    • Flavin-containing amine oxidase domain-containing protein 2
    • KDM 1
    • KDM1
    • Kdm1a
    • KDM1A_HUMAN
    • KIAA0601
    • LSD 1
    • LSD1
    • Lysine (K) specific demethylase 1
    • Lysine (K) specific demethylase 1A
    • Lysine demethylase 1A
    • Lysine specific histone demethylase 1
    • Lysine specific histone demethylase 1A
    • Lysine-specific demethylase 1
    • Lysine-specific demethylase 1A
    • Lysine-specific histone demethylase 1A
    see all
  • 機能
    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • ドメイン
    The SWIRM domain may act as an anchor site for a histone tail.
  • 細胞内局在
    Nucleus.
  • Information by UniProt

画像

  • SDS-PAGE analysis of ab125649.

参考文献

ab125649 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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