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Our Abpromise guarantee covers the use of ab42619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Activity: > 118 pmol/min/ug and is lot specific. If you would like more information about the activity of a specific lot, please contact our scientific support team who will be happy to help.
One unit defined as the amount of enzyme that will transfer 1nmol phosphate to Tyr substrate per minute at pH 7.4 and 30deg.C. Assay buffer : 50mM HEPES pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3um Na-orthovanadate, 0.1M ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate and 4ug/ml recombinant JAK2.
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.