Recombinant human Insulin protein (ab73797)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Human
    • 分子量6 kDa
    • 配列の追加情報ab73797 is a two chain (aa 90-110 insulin A, aa 25-54 insulin B), non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton.

特性

Our Abpromise guarantee covers the use of ab73797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    28 units/mg

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 95 % SDS-PAGE.
    ab73797 is purified by proprietary chromatographic techniques. Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
  • 製品の状態Lyophilised
  • 備考

    Lyophilized Insulin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Insulin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

     

    Two chain, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton consisting of human insulin A (21 aa) and B (30 aa) chains

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Please see notes section.

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Reconstitute the lyophilized Insulin in sterile 0.005N HCl to not more than 1 mg/ml.

関連情報

  • 別名
    • IDDM
    • IDDM1
    • IDDM2
    • ILPR
    • ins
    • INS_HUMAN
    • Insulin A chain
    • Insulin B chain
    • IRDN
    • MODY10
    • Preproinsulin
    • Proinsulin
    • Proinsulin precursor
    see all
  • 機能Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 関連疾患Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 配列類似性Belongs to the insulin family.
  • 細胞内局在Secreted.
  • Information by UniProt

Recombinant human Insulin protein (ab73797) 使用論文

ab73797 has not yet been referenced specifically in any publications.

Product Wall

We do offer a number of active insulin proteins for use as standard including http://www.abcam.com/insulin-protein-active-ab73797.html (25mg size) or http://www.abcam.com/insulin-protein-active-ab123768.html(5mg size). Abcam also carries a number of...

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DISCOUNT CODE: xxx for testing ab79477 in ELISA and sELISA
DISCOUNT CODE: xxx for testing ab65454 in sELISA
DISCOUNT CODE: xxx for testing ab73797 in ELISA and sELISA

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Thank you for contacting us. You can ask any questions to us we are happy to help. We can only guarantee these proteins acting as inducers in functional assays in human cells. These proteins are tested only with human cells. The RAT insulin 1, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"