Recombinant human IL-6R protein (ab167742)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human IL-6R protein
IL-6R タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 15-60 ng/ml. -
精製度
> 90 % SDS-PAGE.
ab167742 was lyophilized from 0.22 µm filtered solution. Purified by Immobilized metal affinity chromatography. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
LAPRRCPAQEVARGVLTSLPGDSVTLTCPGVEPEDNATVHWVLRKPAAGS HPSRWAGMGRRLLLRSVQLHDSGNYSCYRAGRPAGTVHLLVDVPPEEPQL SCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQ ESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGCGILQPDPP ANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWM VKDLQHHCVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESR SPPAENEVSTPMQALTTNKDDDNILFRDSANATSLPVQDSSSVPLP -
予測される分子量
40 kDa including tags -
領域
20 to 365 -
タグ
His tag N-Terminus
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関連製品
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Positive Controls
特性
Our Abpromise guarantee covers the use of ab167742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 94% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 250 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
関連情報
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別名
- CD 126
- CD126
- CD126 antigen
see all -
機能
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity. -
組織特異性
Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum. -
配列類似性
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
ドメイン
The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. -
翻訳後修飾
A short soluble form may also be released from the membrane by proteolysis. -
細胞内局在
Secreted and Basolateral cell membrane. - Information by UniProt
画像
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Human IL-6 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
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Immobilized ActiveMax® Human IL-6, Tag Free at 5 µg/mL (100 µL/well) can bind Human IL-6 R alpha, His Tag with a linear range of 1-16 ng/mL.
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SDS-PAGE analysis of reduced ab167742 and staining overnight with Coomassie Blue. Protein migrates as 60-70 kDa in reduced SDS-PAGE resulting from glycosylation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab167742 は 2 報の論文で使用されています。
- Villar-Fincheira P et al. Soluble Interleukin-6 Receptor Regulates Interleukin-6-Dependent Vascular Remodeling in Long-Distance Runners. Front Physiol 12:722528 (2021). PubMed: 34707507
- Sun Q et al. Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Cell Invasion through Integrin ß6 Upregulation in Colorectal Cancer. Oxid Med Cell Longev 2020:8032187 (2020). PubMed: 32855767