Recombinant human IL-6 protein (Active) (ab119444)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: WB, ELISA, Functional Studies, SDS-PAGE, HPLC
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品の詳細
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製品名
Recombinant human IL-6 protein (Active)
IL-6 タンパク質・ペプチド 製品一覧 -
生理活性
>1 x 107 units/mg.
ab119444 ED50 is 0.1ng/ml when assayed by proliferation of murine 7TD1 cells. -
精製度
> 98 % SDS-PAGE.
>98% HPLC analyes. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM -
予測される分子量
21 kDa -
領域
29 to 212
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特性
Our Abpromise guarantee covers the use of ab119444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
ELISA
Functional Studies
SDS-PAGE
HPLC
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成5 ug size: Reconstitute with 50ul distilled water. 20 ug size: Reconstitute with 200ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. DO NOT VORTEX. For long term storage the addition of 0.1% bovine serum albumin (BSA) is recommended. N.B. For functional studies, do not add sodium azide.
関連情報
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別名
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
機能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
関連疾患
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab119444 は 2 報の論文で使用されています。
- Chen J et al. Early detection of Alzheimer's disease by peptides from phage display screening. Brain Res 1721:146306 (2019). PubMed: 31247207
- Kasprzycka P et al. The factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts. Stem Cell Res Ther 10:343 (2019). PubMed: 31753006