Recombinant Human IL5RA protein (Fc Chimera) (ab83828)

製品の概要

• 製品名Recombinant Human IL5RA protein (Fc Chimera)
• タンパク質長Protein fragment

製品の詳細

• 由来Recombinant
• 由来HEK 293 cells
• アミノ酸配列
  • アクセッション番号Q01344
  • 生物種Human
  • 配列DLLPDEKISLLPPVNFTIKVTGLAQVLLQWKPNPDQEQRNVNLEYQVKIN APKEDDYETRITESKCVTILHKGFSASVRTILQNDHSLLASSWASAELHA PPGSPGTSIVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGTDAPEDTQYF LYYRYGSWTEECQEYSKDTLGRNIACWFPRTFILSKGRDWLAVLVNGSSK HSAIRPFDQLFALHAIDQINPPLNVTAEIEGTRLSIQWEKPVSAFPIHCF DYEVKIHNTRNGYLQIEKLMTNAFISIIDDLSKYDVQVRAAVSSMCREAG LW
  • 分子量62 kDa including tags
  • 領域21 to 322
  • 配列の追加情報Chimeric Protein: A DNA sequence (aa 21-322) was fused to the Fc region of human IgG1 (aa 93-330).

特性

• アプリケーション

  SDS-PAGE

• 精製度>95% by SDS-PAGE .
  
  
• 製品の状態Lyophilised
• 備考

  Molecular mass: Chimera migrates as a broad band between 70 and 80 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares

  with the unmodified Chimera that has a predicted molecular mass of 62.1 kDa. The final cellular processed form of the protein is expected to be 301 amino acids in length.

  pI: Chimera separates into a number of isoforms with a pI between 5.8 and 7.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Chimera that has a predicted pI of 6.34.

  Chimera consists of 10-25% carbohydrate by weight.

  Chimera has N-linked and O-linked oligosaccharides.

• Concentration information loading...

前処理および保存

• 保存方法および安定性

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Reconstitute for long term storage.

  Constituents: 89% PBS, 10% Trehalose, 1% Human serum albumin

• 再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

Recombinant Human IL5RA protein (Fc Chimera) 画像

• 

  SDS-PAGE - IL5RA protein (Fc Chimera) (ab83828)
  1D SDS-PAGE of ab83828 before and after treatment with glycosidases to remove oligosaccharides.
  Lane 1: ab83828
  Lane 2: ab83828 treated with PNGase F to remove potential N-linked glycans
  Lane 3: ab83828 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
  10 μg protein loaded per lane; Deep Purple™ stained.
• 

  SDS-PAGE - IL5RA protein (Fc Chimera) (ab83828)
  A sample of ab83828 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein loaded per lane; Deep Purple™ stained.
• 

  SDS-PAGE - Human IL5RA protein fragment (ab83828)
  Densitometry of protein isoforms visualised by 2-DE.
  The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
  The triangle indicates the theoretical MW and pI of the protein.