Recombinant Human IL5RA protein (Fc Chimera) (ab83828)

製品の概要

  • 製品名Recombinant Human IL5RA protein (Fc Chimera)
  • タンパク質長Protein fragment

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • アクセッション番号Q01344
    • 生物種Human
    • 配列DLLPDEKISLLPPVNFTIKVTGLAQVLLQWKPNPDQEQRNVNLEYQVKIN APKEDDYETRITESKCVTILHKGFSASVRTILQNDHSLLASSWASAELHA PPGSPGTSIVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGTDAPEDTQYF LYYRYGSWTEECQEYSKDTLGRNIACWFPRTFILSKGRDWLAVLVNGSSK HSAIRPFDQLFALHAIDQINPPLNVTAEIEGTRLSIQWEKPVSAFPIHCF DYEVKIHNTRNGYLQIEKLMTNAFISIIDDLSKYDVQVRAAVSSMCREAG LW
    • 分子量62 kDa including tags
    • 領域21 to 322
    • 配列の追加情報Chimeric Protein: A DNA sequence (aa 21-322) was fused to the Fc region of human IgG1 (aa 93-330).

関連製品

特性

Our Abpromise guarantee covers the use of ab83828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度>95% by SDS-PAGE .

  • 製品の状態Lyophilised
  • 備考

    Molecular mass: Chimera migrates as a broad band between 70 and 80 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares

    with the unmodified Chimera that has a predicted molecular mass of 62.1 kDa. The final cellular processed form of the protein is expected to be 301 amino acids in length.

    pI: Chimera separates into a number of isoforms with a pI between 5.8 and 7.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Chimera that has a predicted pI of 6.34.

    Chimera consists of 10-25% carbohydrate by weight.

    Chimera has N-linked and O-linked oligosaccharides.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Reconstitute for long term storage.

    Constituents: 89% PBS, 10% Trehalose, 1% Human serum albumin

  • 再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

関連情報

  • 別名
    • CD125
    • CD125 antigen
    • CDw125
    • HSIL5R3
    • IL-5 receptor subunit alpha
    • IL-5R subunit alpha
    • IL-5R-alpha
    • IL-5RA
    • IL5R
    • IL5RA
    • IL5RA_HUMAN
    • Interleukin 5 Receptor alpha
    • Interleukin 5 Receptor alpha chain
    • Interleukin 5 receptor alpha subunit
    • Interleukin 5 receptor subunit alpha
    • Interleukin 5 receptor type 3
    • Interleukin-5 receptor subunit alpha
    • MGC26560
    see all
  • 機能This is the receptor for interleukin-5. The alpha chain binds to IL5.
  • 組織特異性Expressed on eosinophils and basophils.
  • 配列類似性Belongs to the type I cytokine receptor family. Type 5 subfamily.
  • ドメインThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 細胞内局在Membrane.
  • Information by UniProt

Recombinant Human IL5RA protein (Fc Chimera) 画像

  • 1D SDS-PAGE of ab83828 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab83828
    Lane 2: ab83828 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab83828 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
    10 μg protein loaded per lane; Deep Purple™ stained.
  • A sample of ab83828 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein loaded per lane; Deep Purple™ stained.
  • Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MW and pI of the protein.

Recombinant Human IL5RA protein (Fc Chimera) (ab83828) 使用論文

ab83828 has not yet been referenced specifically in any publications.

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