The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
精製度>95% by SDS-PAGE .
Molecular mass: Chimera migrates as a broad band between 70 and 80 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares
with the unmodified Chimera that has a predicted molecular mass of 62.1 kDa. The final cellular processed form of the protein is expected to be 301 amino acids in length.
pI: Chimera separates into a number of isoforms with a pI between 5.8 and 7.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Chimera that has a predicted pI of 6.34.
Chimera consists of 10-25% carbohydrate by weight.
Chimera has N-linked and O-linked oligosaccharides.
Concentration information loading...
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Reconstitute for long term storage.
Constituents: 89% PBS, 10% Trehalose, 1% Human serum albumin
再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
IL-5 receptor subunit alpha
IL-5R subunit alpha
Interleukin 5 Receptor alpha
Interleukin 5 Receptor alpha chain
Interleukin 5 receptor alpha subunit
Interleukin 5 receptor subunit alpha
Interleukin 5 receptor type 3
Interleukin-5 receptor subunit alpha
機能This is the receptor for interleukin-5. The alpha chain binds to IL5.
組織特異性Expressed on eosinophils and basophils.
配列類似性Belongs to the type I cytokine receptor family. Type 5 subfamily.
ドメインThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
1D SDS-PAGE of ab83828 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83828
Lane 2: ab83828 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83828 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10 μg protein loaded per lane; Deep Purple™ stained.
SDS-PAGE - IL5RA protein (Fc Chimera) (ab83828)
A sample of ab83828 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein loaded per lane; Deep Purple™ stained.
SDS-PAGE - Human IL5RA protein fragment (ab83828)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangle indicates the theoretical MW and pI of the protein.
Recombinant Human IL5RA protein (Fc Chimera) (ab83828) 使用論文
has not yet been referenced specifically in any publications.