Recombinant human IL4R protein (Fc Chimera) (ab83756)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • アクセッション番号P24394
    • 生物種Human
    • 配列Theoretical Sequence: MKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQL VFLLSEAHTCIPEN NGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGN L TVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTY LEP SLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQH G SSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR TPE VTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTV LH QDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTK NQV SLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVD KSR WQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    • 領域1 to 232
    • 配列の追加情報A DNA sequence encoding the signal peptide and extracellular domain of human IL-4 R alpha (aa 1-232) was fused to the Fc region of human IgG1 (aa 90-330). Protein expressed in modified human 293 cells

特性

Our Abpromise guarantee covers the use of ab83756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性The ED50 of IL4R - Fc Chimera is typically 45-60 ng/ml as measured by its ability to neutralize IL-4 mediated proliferation of the human growth factor dependent TF-1 cell line.
  • アプリケーション

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • 備考


    ab83756 consists of 15-35% carbohydrate by weight. ab83756 contains N-linked oligosaccharides and may have O-linked oligosaccharides.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.

関連情報

  • 別名
    • CD124
    • IL 4R alpha
    • IL-4 receptor subunit alpha
    • IL-4-binding protein
    • IL-4R subunit alpha
    • IL-4R-alpha
    • IL-4RA
    • IL4-BP
    • Il4r
    • IL4R nirs variant 1
    • IL4RA
    • IL4RA_HUMAN
    • Interleukin 4 receptor
    • Interleukin 4 receptor alpha chain
    • sIL4Ralpha/prot
    • Soluble IL-4 receptor subunit alpha
    • Soluble IL-4R-alpha
    • Soluble interleukin-4 receptor subunit alpha
    see all
  • 機能Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
    Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
  • 組織特異性Isoform 1 and isoform 2 are highly expressed in activated T-cells.
  • 配列類似性Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.
  • ドメインThe extracellular domain represents the IL4 binding protein (IL4BP).
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • 翻訳後修飾On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.
    The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt

Recombinant human IL4R protein (Fc Chimera) 画像

  • Lane 1: MW markers; Lane 2: IL4R - Fc Chimera; Lane 3: IL4R - Fc Chimera treated with PNGase F to remove potential N-linked glycans; Lane 4: IL4R - Fc Chimera treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie.
    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
  • Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins.
    The triangle indicates theoretical pI and MW of the protein.

Recombinant human IL4R protein (Fc Chimera) (ab83756) 使用論文

ab83756 has not yet been referenced specifically in any publications.

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