Recombinant Human IL3RA protein (Fc Chimera) (ab88358)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • アクセッション番号P26951
    • 生物種Human
    • 配列TKEDPNPPITNLRMKAKAQQLTWDLNRNVTDIECVKDADYSMPAVN NS YCQFGAISLCEVTNYTVRVANPPFSTWILFPENSGKPWAGAENL TCWI HDVDFLSCSWAVGPGAPADVQYDLYLNVANRRQQYECLHYKT DAQGTR IGCRFDDISRLSSGSQSSHILVRGRSAAFGIPCTDKFVVF SQIEILTP PNMTAKCNKTHSFMHWKMRSHFNRKFRYELQIQKRMQP VITEQVRDRT SFQLLNPGTYTVQIRARERVYEFLSAWSTPQRFECD QEEGANTRGGRV DGIQWIPKVDKKVEPKSCDKTHTCPPCPAPELLG GPSVFLFPPKPKDT LMISRTPEVTCVVVDVSHEDPEVKFNWYVDGV EVHNAKTKPREEQYNS TYRVVSVLTVLHQDWLNGKEYKCRVSNKAL PAPIEKTISKAKGQPREP QVYTLPPSRDELTKNQVSLTCLVKGFYP SDIAVEWESNGQPENNYKTT PPVLDSDGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKSLS LSPGK
    • 領域19 to 302
    • 配列の追加情報DNA sequence encoding the signal peptide and extracellular domain of human IL3R alpha (aa 1-18) was fused to the Fc region of human IgG1 (aa 93-330). Protein expressed in modified human 293 cells.

特性

Our Abpromise guarantee covers the use of ab88358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

  • 再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

関連情報

  • 別名
    • CD123
    • CD123 antigen
    • hIL 3Ra
    • hIL-3Ra
    • hIL3Ra
    • IL 3 receptor alpha SP2 isoform
    • IL 3R alpha
    • IL-3 receptor subunit alpha
    • IL-3R subunit alpha
    • IL-3R-alpha
    • IL-3RA
    • IL3 Receptor alpha
    • IL3R
    • Il3ra
    • IL3RA_HUMAN
    • IL3RAX
    • IL3RAY
    • IL3RX
    • IL3RY
    • Interleukin 3 receptor alpha chain
    • Interleukin 3 receptor, alpha
    • Interleukin 3 receptor, alpha (low affinity)
    • Interleukin-3 Receptor alpha
    • Interleukin-3 receptor subunit alpha
    • Interleukin3 receptor
    • Interleukin3 receptor, Y-chromosomal
    see all
  • 機能This is a receptor for interleukin-3.
  • 配列類似性Belongs to the type I cytokine receptor family. Type 5 subfamily.
  • ドメインThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 細胞内局在Membrane.
  • Information by UniProt

Recombinant Human IL3RA protein (Fc Chimera) 画像

  • 1D SDS-PAGE of ab88358 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab88358
    Lane 2: ab88358 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab88358 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.

    Drop in MWt after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MWt after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
  • A sample of ab88358 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train indicates presence of multiple isoforms of ab88358.
  • Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MWt and pI of the protein.

Recombinant Human IL3RA protein (Fc Chimera) (ab88358) 使用論文

ab88358 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab88358.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"