The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
IL 3 receptor alpha SP2 isoform
IL 3R alpha
IL-3 receptor subunit alpha
IL-3R subunit alpha
IL3 Receptor alpha
Interleukin 3 receptor alpha chain
Interleukin 3 receptor, alpha
Interleukin 3 receptor, alpha (low affinity)
Interleukin-3 Receptor alpha
Interleukin-3 receptor subunit alpha
Interleukin3 receptor, Y-chromosomal
This is a receptor for interleukin-3.
Belongs to the type I cytokine receptor family. Type 5 subfamily.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
1D SDS-PAGE of ab88358 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab88358
Lane 2: ab88358 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab88358 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Drop in MWt after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MWt after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
SDS-PAGE - IL3RA protein (Fc Chimera) (ab88358)
A sample of ab88358 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train indicates presence of multiple isoforms of ab88358.
Functional Studies - IL3RA protein (Fc Chimera) (ab88358)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangle indicates the theoretical MWt and pI of the protein.
Recombinant Human IL3RA protein (Fc Chimera) (ab88358) 使用論文
has not yet been referenced specifically in any publications.