Recombinant human IL-31 protein (ab106619)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, ELISA, WB
製品の詳細
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製品名
Recombinant human IL-31 protein
IL-31 タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its ability to induce STAT3 activation in Human U87 cells. -
精製度
> 98 % SDS-PAGE.
Greater than 98% by SDS-PAGE gel and HPLC analyses -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T -
予測される分子量
16 kDa -
領域
24 to 164
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab106619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
ELISA
Western blot
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成The lyophilised product should be stored at -20oC till use. Once the product has been reconstituted according to the CoA, this can be diluted further in a chosen aqueous buffer containing a carrier protein, such as 0.1% BSA and stored in working aliquots at -20oC for at least 3 months
関連情報
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別名
- IL 31
- IL-31
- IL31
see all -
機能
Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. -
組織特異性
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. -
細胞内局在
Secreted. - Information by UniProt
画像
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Whole cell lysate prepared from human eosinophil granulocytes was loaded at 500000 cells.
5-16% gel run under reducing, denaturing conditions.Blocking step performed using 5% BSA for 2 hours at 25°C.
ab106619 used at a 1/200 dilution.Primary antibody used at a 1/100 dilution.
Secondary antibody was an HRP conjugated goat anti-rabbit IgG, used at a 1/200 dilution.The positive control was stimulated with IL-3 (10ng/ml) + IL-5 (10ng/ml) + GM-CSF (10ng/ml) for 30 minutes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab106619 は論文での使用が確認できていません。