Recombinant human IL-23 protein (ab106889)
Key features and details
- Expression system: BTI-TN-5B1-4 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human IL-23 protein
IL-23 タンパク質・ペプチド 製品一覧 -
生理活性
Biological activity measured by the ability to induce IL-17 secretion by mouse splenocytes.
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精製度
> 95 % SDS-PAGE.
Purity greater than 95% by SDS-PAGE gel and HPLC analyses. Sterile filtered through a 0.2 micron filter. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
BTI-TN-5B1-4 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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アミノ酸配列 1
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生物種
Human -
配列
RAVPGGSSPA WTQCQQLSQK LCTLAWSAHP LVGHMDLREE GDEETTNDVP HIQCGDGCDP QGLRDNSQFC LQRIHQGLIF YEKLLGSDIF TGEPSLLPDS PVGQLHASLL GLSQLLQPEG HHWETQQIPS LSPSQPWQRL LLRFKILRSL QAFVAVAARV FAHGAATLSP -
予測される分子量
54 kDa -
配列の追加情報
Recombinant Human IL-23 is a heterodimeric protein consisting of the two subunits, p19 (170 amino acids) and p40 (306 amino acids).
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アミノ酸配列 2
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生物種
Human -
配列
IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS -
予測される分子量
54 kDa -
配列の追加情報
Recombinant Human IL-23 is a heterodimeric protein consisting of the two subunits, p19 (170 amino acids) and p40 (306 amino acids).
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関連製品
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Positive Controls
特性
Our Abpromise guarantee covers the use of ab106889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
備考
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
Constituent: 2.68% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
関連情報
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別名
- IL 23
- IL 23 A
- IL 23 subunit alpha
see all -
機能
Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
組織特異性
Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level). -
配列類似性
Belongs to the IL-6 superfamily. -
発生段階
Expressed by newborns dendritic cells. -
細胞内局在
Secreted. Secreted upon association with IL12B. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab106889 は 1 報の論文で使用されています。
- Iwata H et al. Possible role of epiregulin from dermal fibroblasts in the keratinocyte hyperproliferation of psoriasis. J Dermatol 48:1433-1438 (2021). PubMed: 34128258