The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Activity was measured by its ability to stimulate the proliferation of PHA-activated human T-lymphoblasts, and is typically in the range of 0.05-0.20 ng/ml.
Concentration information loading...
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
1D SDS-PAGE of ab89013 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab89013
Lane 2: ab89013 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab89013 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Appearance of additional band at lower MWt after treatment with PNGase F indicates the presence of N-linked glycans. The subsequent drop in MWt after treatment with a glycosidase cocktail indicates the presence of O-linked glycans. Additional high MWt bands in lane 3 are glycosidase enzymes.
SDS-PAGE - IL12 protein (Active) (ab89013)
A sample of ab89013 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg of protein was loaded. Spot trains indicate presence of multiple isoforms.
Functional Studies - IL12 protein (Active) (ab89013)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangles indicate theoretical pI and MWt of the alpha and beta chains of the protein.
has not yet been referenced specifically in any publications.