Recombinant Human IL1 Receptor I protein (Fc Chimera) (ab83575)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来HEK 293 cells
  • アミノ酸配列
    • アクセッション番号P14778
    • 生物種Human
    • 配列Theoretical Sequence: LEADKCKEREEKIILVSSANEIDVRPCPLNPNEHKGTIT WYKDDSKTP VSTEQASRIHQHKEKLWFVPAKVEDSGHYYCVVRNSSYC LRIKISAKF VENEPNLCYNAQAIFKQKLPVAGDGGLVCPYMEFFKNEN NELPKLQWY KDCKPLLLDNIHFSGVKDRLIVMNVAEKHRGNYTCHASY TYLGKQYPI TRVIEFITLEENKPTRPVIVSPANETMEVDLGSQIQLIC NVTGQLSDI AYWKWNGSVIDEDDPVLGEDYYSVENPANKRRSTLITVL NISEIESRF YKHPFTCFAKNTHGIDAAYIQLIYPVTNRSSNTKVDKKV EPKSCDKTH TCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVV VDVSHEDPE VKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQ DWLNGKEYK CRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELT KNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLY SKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    • 領域18 to 333
    • 配列の追加情報A DNA sequence encoding the signal peptide and extracellular domain of human IL-1RI (aa 1-333) was fused to the Fc region of human IgG1 (aa 93-330). Protein expressed in modified human 293 cells.

関連製品

特性

Our Abpromise guarantee covers the use of ab83575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

  • 再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

関連情報

  • 別名
    • Antigen CD121a
    • CD121 antigen-like family member A
    • CD121a
    • CD121a antigen
    • D2S1473
    • IL 1
    • IL 1R 1
    • IL 1R alpha
    • IL 1RT1
    • IL-1R-1
    • IL-1R-alpha
    • IL-1RT-1
    • IL-1RT1
    • IL1 inhibitor
    • IL1R
    • IL1R1
    • IL1R1_HUMAN
    • IL1RA
    • IL1RN
    • IL1RT1
    • Interleukin 1 receptor alpha type 1
    • Interleukin 1 receptor antagonist protein (IRAP)
    • Interleukin 1 receptor type I
    • Interleukin receptor 1
    • Interleukin-1 receptor alpha
    • Interleukin-1 receptor type 1
    • Interleukin-1 receptor type I
    • p80
    see all
  • 機能Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
  • 配列類似性Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • 細胞内局在Membrane.
  • Information by UniProt

Recombinant Human IL1 Receptor I protein (Fc Chimera) 画像

  • Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
  • 1D SDS-PAGE of ab83575 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1 – MW markers; Lane 2 – ab83575; Lane 3 – ab83575 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83575 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein loaded per lane.

    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.

Recombinant Human IL1 Receptor I protein (Fc Chimera) (ab83575) 使用論文

ab83575 has not yet been referenced specifically in any publications.

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