Recombinant human IGFBP1 protein (ab50216)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human IGFBP1 protein
IGFBP1 タンパク質・ペプチド 製品一覧 -
生理活性
Biological Activity : The ED50, as determined by its ability to inhibit IGF-I induced proliferation of MCF-7, is < 0.5 µg/ml in the presence of 6 ng/ml of human IGF-I.
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精製度
> 98 % SDS-PAGE.
Greater than 98% by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g). -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN
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特性
Our Abpromise guarantee covers the use of ab50216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconsitute to 1mg/ml with sterile water.
関連情報
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別名
- AFBP
- Alpha pregnancy associated endometrial globulin
- Amniotic fluid binding protein
see all -
機能
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. -
配列類似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
翻訳後修飾
Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs. -
細胞内局在
Secreted. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab50216 は論文での使用が確認できていません。