Recombinant Human HUS1 protein (ab101944)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号O60921
    • 生物種Human
    • 配列MGSSHHHHHHSSGLVPRGSHMKFRAKIVDGACLNHFTRISNMIAKLAKTC TLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIY LELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVT HDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHL VIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEV HIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS
    • 分子量34 kDa including tags
    • 領域1 to 280
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab101944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Mass Spectrometry

  • 質量分析
    MALDI-TOF
  • 精製度> 95 % SDS-PAGE.
    ab101944 is purified using conventional chromatography techniques.
  • 製品の状態Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 40% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, pH 8.0

関連情報

  • 別名
    • Checkpoint protein HUS1
    • hHUS1
    • Hus1
    • HUS1 (S. pombe) checkpoint homolog
    • HUS1 checkpoint homolog (S. pombe)
    • HUS1 Checkpoint Protein
    • HUS1 protein
    • HUS1+ - like protein
    • HUS1_HUMAN
    • Hydroxyurea-sensitive 1, S. pombe, homolog of
    see all
  • 機能Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the HUS1 family.
  • 細胞内局在Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt

Recombinant Human HUS1 protein 画像

  • 15% SDS-PAGE analysis of 3µg ab101944.

Recombinant Human HUS1 protein (ab101944) 使用論文

ab101944 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and I am sorry for the delay in getting back to you.

As mentioned in my previous email I have contacted the lab in regards to the HUS1 protein, ab101944 in order to try to understand why the results you have observe...

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Thank you for your reply and for sharing that extra information.

From the details provided by the customer I cannot explain why they are not able to see the protein by SDS-PAGE when staining with the Coomassie stain. If they would like, I can ...

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I am sorry for the delay in getting back to you. I now have further information to share with you from the lab in regards to the HUS1 protein (ab101944).

Following the concerns raised by you, the protein has been re-run on an SDS-PAGE gel to o...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In orde...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"