Recombinant human Hsp90 beta protein (ab80033)

製品の概要

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected BTI-TN-5B1-4 cells
  • アミノ酸配列
    • 生物種
      Human

特性

Our Abpromise guarantee covers the use of ab80033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    1. The reactions are in 33 mM Hepes pH7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µl reaction at 37ºC. It is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.

    2. The protein concentrations (where applicable) were 2 µM Hsp90 (SPR-102 Hsp90 beta), 2 µM Ahsa1 (His tagged human Ahsa1), 4 µM p23 (cleaved human p23), 4 µM HOP (His-tagged human HOP).

    3. Addition of a two-fold excess of p23 reduced Aha1-mediated Hsp90 stimulation by 30% (when using 2 µM Hsp90 and Aha1).

    4. Addition of a two -fold excess of HOP completely eliminated Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity.

  • アプリケーション

    Western blot

    ELISA

    Inhibition Assay

    Functional Studies

    SDS-PAGE

  • 精製度
    > 90 % SDS-PAGE.
    ab80033 is Multi-step chromatographically purified.
  • 製品の状態
    Liquid
  • 備考
    ab80033 is endotoxin free. <0.1 EU/ml.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris, 175mM Sodium chloride, 1mM DTT, 0.1mM EDTA, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • 90 kda heat shock protein beta HSP90 beta
    • D6S182
    • FLJ26984
    • Heat shock 84 kDa
    • Heat shock 90kD protein 1, beta
    • Heat shock 90kDa protein 1 beta
    • Heat shock protein 90 alpha family class B member 1
    • Heat shock protein 90 kDa
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1
    • Heat shock protein 90kDa alpha family class B member 1
    • Heat shock protein beta
    • Heat shock protein HSP 90 beta
    • Heat shock protein HSP 90-beta
    • HS90B_HUMAN
    • HSP 84
    • HSP 90
    • HSP 90 b
    • HSP 90b
    • HSP84
    • HSP90 BETA
    • hsp90ab1
    • HSP90B
    • HSPC2
    • HSPCB
    see all
  • 機能
    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • 配列類似性
    Belongs to the heat shock protein 90 family.
  • ドメイン
    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • 翻訳後修飾
    Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity.
  • 細胞内局在
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

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  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

参考文献

ab80033 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us. The His tags for all four proteins you asked about are all 6X tags. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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