Recombinant human Hsp70 protein (ab78433)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, ELISA, Functional Studies, Competitive Binding Assays, SDS-PAGE
製品の詳細
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製品名
Recombinant human Hsp70 protein
Hsp70 タンパク質・ペプチド 製品一覧 -
生理活性
ab78433 has ATPase activity at the time of manufacture of 3.0µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
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精製度
> 95 % SDS-PAGE. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
AKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLI GDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKP KVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFN DSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLG GGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKK DISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRAR FEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQ DFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSL GLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTK DNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKI TITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKS AVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQ VCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD -
予測される分子量
70 kDa -
領域
1 to 641
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab78433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
ELISA
Functional Studies
Competitive Binding Assays
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Constituents: 0.242% Tris, 0.0154% DTT, 0.0475% Magnesium chloride, 0.375% Potassium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- DnaK type molecular chaperone HSP70 1
- Epididymis secretory protein Li 103
- FLJ54303
see all -
関連性
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
細胞内局在
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab78433 は 1 報の論文で使用されています。
- Goldberg-Murow M et al. Autoantibodies Against Ubiquitous and Confined Antigens in Patients With Ocular, Neuro-Ophthalmic and Congenital Cerebral Toxoplasmosis. Front Immunol 12:606963 (2021). PubMed: 34054794