The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Lyophilized Hsp22 should be stored desiccated below 0oC. Upon reconstitution Hsp22 can be stored at 4oC for 2-7 days. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA).
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Centrifuge the vial prior to opening.
Reconstitute in H20 to a concentration of 0.1 - 1.0 mg/ml.