製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 配列
      MGSSHHHHHHSSGLVPRGSHMDPKYQRVELNDGHFMPVLGFGTYAPPEVP RNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIF YTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLP KDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNCRQLEMILNKP GLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDP NSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIREN IQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY
    • 分子量
      39 kDa including tags
    • 領域
      1 to 343
    • タグ
      His tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab109831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    Specific activity is > 700 pmol/min/μg, and is defined as the amount of enzyme that catalyzes the reduction of 1.0 pmole 3-chlorobenzaldehyde in the presence of NADP per minute at pH 8.8 at 25°C.

  • アプリケーション

    Functional Studies

    SDS-PAGE

    Mass Spectrometry

  • 質量分析
    MALDI-TOF
  • 精製度
    > 90 % SDS-PAGE.
    ab109831 was purified using conventional chromatography.
  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% DTT, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • 3 alpha HSD1
    • 3-alpha-HSD1
    • 3-alpha-hydroxysteroid dehydrogenase type I
    • AK1C4_HUMAN
    • AKR1C4
    • Aldo keto reductase family 1 member C4
    • Aldo-keto reductase family 1 member C4
    • CDR
    • Chlordecone reductase
    • DD-4
    • DD4
    • Dihydrodiol dehydrogenase 4
    • HAKRA
    see all
  • 機能
    Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20-alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route.
  • 組織特異性
    Liver specific.
  • 配列類似性
    Belongs to the aldo/keto reductase family.
  • 翻訳後修飾
    The N-terminus is blocked.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt

Recombinant human HSD3a protein 画像

  • 15% SDS-PAGE image, using ab109831 at 3ug

Recombinant human HSD3a protein (ab109831) 使用論文

ab109831 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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