Recombinant Human HLA-DQA1 protein (ab114250)

製品の概要

  • 製品名Recombinant Human HLA-DQA1 protein
  • タンパク質長Protein fragment

製品の詳細

  • 由来Recombinant
  • 由来Wheat germ
  • アミノ酸配列
    • アクセッション番号P01909
    • 生物種Human
    • 配列EDIVADHVASCGVNLYQFYGPSGQYTHEFDGDEQFYVDLERKETAWRWPE FSKFGGFDPQGALRNMAVAKHNLNIMIKRYNSTAATN
    • 分子量35 kDa including tags
    • 領域24 to 110

特性

Our Abpromise guarantee covers the use of ab114250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    ELISA

    SDS-PAGE

    Western blot

  • 製品の状態Liquid
  • 備考Protein concentration is above or equal to 0.05 µg/µl
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

関連情報

  • 別名
    • CD
    • CELIAC1
    • DC 1 alpha chain
    • DC alpha
    • DC-1 alpha chain
    • DC-alpha
    • DC1, included
    • DQ alpha 1 chain
    • DQ-A1
    • DQ-DRW9 alpha chain
    • DQA1_HUMAN
    • FLJ27088
    • FLJ27328
    • Gluten-sensitive enteropathy (celiac disease)
    • GSE
    • HLA class II histocompatibility antigen
    • HLA class II histocompatibility antigen, DQ alpha 1 chain
    • HLA class II histocompatibility antigen, DQ(W3) alpha chain
    • HLA-DCA
    • HLA-DQA
    • HLA-DQA1
    • HLA-DQA1 major histocompatibility complex, class II, DQ alpha 1
    • HLADC histocompatibility type
    • Immune response antigens HIa, included
    • leucocyte antigen DQA1
    • leukocyte antigen alpha chain
    • LOC100133678
    • LOC100507686
    • LOC100509457
    • Major histocompatibility complex, class II, DQ alpha 1
    • MGC149527
    • MHC class II antigen
    • MHC class II DQA1
    • MHC class II HLA-D alpha glycoprotein
    • MHC class II HLA-DQ alpha 1
    • MHC class II surface glycoprotein
    • MHC HLA-DQ alpha
    • OTTHUMP00000029141
    • OTTHUMP00000176885
    • OTTHUMP00000178551
    • OTTHUMP00000178552
    • OTTHUMP00000233817
    see all
  • 機能Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • 配列類似性Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt

Recombinant Human HLA-DQA1 protein 画像

  • ab114250 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.

Recombinant Human HLA-DQA1 protein (ab114250) 使用論文

ab114250 has not yet been referenced specifically in any publications.

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